1. Write a description about what you intend to do with automation tools for your final project. You may include example pseudocode or Python scripts, procedures you may need to automate, 3D printed holders you may need, and more.

I wanted to automate the opentrone to make the name KENYA and do it using the colours of our flag but replace those that are not availble with the availableo nes. In order to do this, I had to edit the code that was used to generate the HTGAA image previusly done. Here is the code that was used as the foundational code.

This is the image i am hoping to generate

DALL·E 2025-02-27 00.37.09 - An agar plate with bacterial colonies arranged to spell 'KENYA' in the shape of the Kenyan flag. The word is written in three horizontal stripes_ blac.webp

HTGAA code

In order to achieve this, and after several prompts and editing of the code, This is the code I came up with iniorder to generate a similar results as shown in the AI generated image

“KENYA” code

  1. Find and describe a published paper that utilizes the Opentrons or similar automation tools to achieve novel biological applications (eg automated PACE)

I reviewed a paper titled “Automation of biochemical assays using an open-sourced, inexpensive robotic liquid handler” in this paper evaluates the Opentrons OT-2 liquid handler as a low-cost alternative for High Throughput Screening in pharmaceutical research, particularly in assay development and sample screening. It compares OT-2’s performance to the Tecan EVO, assessing its accuracy, precision, and limitations. The results show that OT-2 provides accurate pipetting with low variance, making it a viable option for early-stage development and method transfer, despite some constraints like limited deck space and the absence of a crash detection system. The study highlights OT-2’s affordability, open-source flexibility, and potential to expand testing capabilities with minimal investment.